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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MOLT-4 cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of MOLT-4 using 68558-1-Ig (same clone as 68558-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

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HELIOS Monoclonal antibody, PBS Only

HELIOS Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 68558-1-PBS
Clone No.1F1F10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68558-1-PBS targets HELIOS in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HELIOS fusion protein Ag4357 相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 2 (Helios)
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC028936
Gene Symbol HELIOS
Gene ID (NCBI) 22807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKS7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).