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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of Jurkat using 68558-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MOLT-4 cells using HELIOS Monoclonal antibody (68558-1-Ig)

    WB analysis of MOLT-4 using 68558-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HELIOS Monoclonal antibody (68558-1-Ig)

    IP experiment of Jurkat using 68558-1-Ig

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

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HELIOS Monoclonal antibody

HELIOS Monoclonal Antibody for WB, IP, ELISA
Cat No. 68558-1-Ig
Clone No.1F1F10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, ELISA

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HELIOS fusion protein Ag4357 相同性解析による交差性が予測される生物種
Full Name IKAROS family zinc finger 2 (Helios)
Calculated molecular weight 500 aa, 57 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC028936
Gene Symbol HELIOS
Gene ID (NCBI) 22807
RRIDAB_3085260
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).

Protocols

Product Specific Protocols
WB protocol for HELIOS antibody 68558-1-IgDownload protocol
IP protocol for HELIOS antibody 68558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols