Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of K-562 using 11955-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11955-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 11955-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 11955-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11955-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 11955-1-AP
IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
IHC staining of rat thymus using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse thymus using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse thymus using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 11955-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11955-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HELLS antibody (11955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HepG2 using 11955-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

HELLS Polyclonal antibody

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11955-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IF	See 3 publications below

Product Information

11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HELLS fusion protein Ag2558 相同性解析による交差性が予測される生物種
Full Name	helicase, lymphoid-specific
Calculated molecular weight	348 aa, 40 kDa, 97 kDa
Observed molecular weight	97 kDa
GenBank accession number	BC031004
Gene Symbol	HELLS
Gene ID (NCBI)	3070
RRID	AB_2117529
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NRZ9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Protocols

Product Specific Protocols
WB protocol for HELLS antibody 11955-1-AP	Download protocol
IHC protocol for HELLS antibody 11955-1-AP	Download protocol
IF protocol for HELLS antibody 11955-1-AP	Download protocol
IP protocol for HELLS antibody 11955-1-AP	Download protocol
FC protocol for HELLS antibody 11955-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nat Commun Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells Authors - Clara Cousu View Article
mouse	WB,IF	Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS Authors - Akeo Shinkai View Article
mouse	WB	Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses Authors - Maja Vukic View Article
mouse	WB, IF	Genome Biol Lsh regulates LTR retrotransposon repression independently of Dnmt3b function. Authors - Donncha S Dunican View Article
human	WB	mSystems Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes Authors - Joseph M. Dybas View Article
		Hum Mol Genet Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals. Authors - Haoyu Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 11955-1-AP

WB analysis of PC-3 using 11955-1-AP

WB analysis of DU 145 using 11955-1-AP

WB analysis of mouse thymus using 11955-1-AP

WB analysis of mouse testis using 11955-1-AP

IP experiment of mouse testis using 11955-1-AP

IHC staining of rat thymus using 11955-1-AP

IHC staining of rat thymus using 11955-1-AP

IHC staining of mouse thymus using 11955-1-AP

IHC staining of mouse thymus using 11955-1-AP

IHC staining of mouse testis using 11955-1-AP

IHC staining of mouse testis using 11955-1-AP

IHC staining of rat testis using 11955-1-AP

IHC staining of rat testis using 11955-1-AP

IF Staining of HepG2 using 11955-1-AP

FC experiment of HepG2 using 11955-1-AP

HELLS Polyclonal antibody

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells

The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS

CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses

Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes

Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.