HELLS Polyclonal antibody
HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 11955-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | mouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IF | See 3 publications below |
Product Information
11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HELLS fusion protein Ag2558 相同性解析による交差性が予測される生物種 |
Full Name | helicase, lymphoid-specific |
Calculated molecular weight | 348 aa, 40 kDa, 97 kDa |
Observed molecular weight | 97 kDa |
GenBank accession number | BC031004 |
Gene symbol | HELLS |
Gene ID (NCBI) | 3070 |
RRID | AB_2117529 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.
Protocols
Product Specific Protocols | |
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WB protocol for HELLS antibody 11955-1-AP | Download protocol |
IHC protocol for HELLS antibody 11955-1-AP | Download protocol |
IF protocol for HELLS antibody 11955-1-AP | Download protocol |
IP protocol for HELLS antibody 11955-1-AP | Download protocol |
FC protocol for HELLS antibody 11955-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses | ||
Genome Biol Lsh regulates LTR retrotransposon repression independently of Dnmt3b function. | ||
mSystems Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes | ||
Hum Mol Genet Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Diana (Verified Customer) (08-18-2022) | In IF the antibody binds specific in the nucleus and has low background, thats great! In WB I have a band as aspected around 100kd but I also have some other smaller unspecific bands. Here I will work on the troubleshooting. Thanks for the corresponding protocolls with buffers and incubation time.
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FH Michelle (Verified Customer) (10-25-2019) | Great antibody for HELLS. Customer support was really helpful when I was troubleshooting how to clean up my western blot. Changed the path of my project since multiple bands for different isoforms of the protein appeared in the cell lines I was invesitgating.
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