HELLS Polyclonal antibody

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11955-1-AP

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid


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Tested Applications

Positive WB detected inK-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELLS fusion protein Ag2558 相同性解析による交差性が予測される生物種
Full Name helicase, lymphoid-specific
Calculated molecular weight 348 aa, 40 kDa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC031004
Gene symbol HELLS
Gene ID (NCBI) 3070
RRIDAB_2117529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Protocols

Product Specific Protocols
WB protocol for HELLS antibody 11955-1-APDownload protocol
IHC protocol for HELLS antibody 11955-1-APDownload protocol
IF protocol for HELLS antibody 11955-1-APDownload protocol
IP protocol for HELLS antibody 11955-1-APDownload protocol
FC protocol for HELLS antibody 11955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells

Authors - Clara Cousu
mouseWB,IF

Nucleic Acids Res

The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS

Authors - Akeo Shinkai
mouseWB

Sci Adv

CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses

Authors - Maja Vukic
mouseWB, IF

Genome Biol

Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.

Authors - Donncha S Dunican
humanWB

mSystems

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes

Authors - Joseph M. Dybas

Hum Mol Genet

Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.

Authors - Haoyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Diana (Verified Customer) (08-18-2022)

In IF the antibody binds specific in the nucleus and has low background, thats great! In WB I have a band as aspected around 100kd but I also have some other smaller unspecific bands. Here I will work on the troubleshooting. Thanks for the corresponding protocolls with buffers and incubation time.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500 (WB), 1:25 (IF)
  • Cell Tissue Type: mouse testis, GC1, NIH3T3
FH

Michelle (Verified Customer) (10-25-2019)

Great antibody for HELLS. Customer support was really helpful when I was troubleshooting how to clean up my western blot. Changed the path of my project since multiple bands for different isoforms of the protein appeared in the cell lines I was invesitgating.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, Jurkats, HAP1, PBMC