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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HELLS Polyclonal ant (CL488-11955)

    IF Staining of HepG2 using CL488-11955

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HELLS antibody (CL488-11955) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated HELLS Polyclonal ant (CL488-11955)

    FC experiment of HepG2 using CL488-11955

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 HELLS Polyclonal Antibody (CL488-11955)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated HELLS Polyclonal antibody

HELLS Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11955

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11955 targets HELLS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELLS fusion protein Ag2558 相同性解析による交差性が予測される生物種
Full Name helicase, lymphoid-specific
Calculated molecular weight 348 aa, 40 kDa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC031004
Gene Symbol HELLS
Gene ID (NCBI) 3070
RRIDAB_3672521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRZ9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HELLS antibody CL488-11955Download protocol
FC protocol for CL Plus 488 HELLS antibody CL488-11955Download protocol
Standard Protocols
Click here to view our Standard Protocols