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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEXA Monoclonal antibody

HEXA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A4F2

Cat no : 60337-1-Ig

Synonyms

HEXA, Hexosaminidase subunit A, TSD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis using 60337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rabbit testis tissue using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis of rabbit testis using 60337-1-Ig

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis of A431 using 60337-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, rabbit testis tissue, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60337-1-Ig targets HEXA in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HEXA fusion protein Ag1854 相同性解析による交差性が予測される生物種
Full Name hexosaminidase A (alpha polypeptide)
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 50-54 kDa, 60-67 kDa
GenBank accession numberBC018927
Gene symbol HEXA
Gene ID (NCBI) 3073
RRIDAB_2881446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Protocols

Product Specific Protocols
WB protocol for HEXA antibody 60337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols