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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEXA Monoclonal antibody, PBS Only

HEXA Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A4F2

Cat no : 60337-1-PBS

Synonyms

HEXA, Hexosaminidase subunit A, TSD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis using 60337-1-Ig (same clone as 60337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit testis tissue using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis of rabbit testis using 60337-1-Ig (same clone as 60337-1-PBS)

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using HEXA Monoclonal antibody (60337-1-Ig)

    WB analysis of A431 using 60337-1-Ig (same clone as 60337-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60337-1-PBS targets HEXA in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HEXA fusion protein Ag1854 相同性解析による交差性が予測される生物種
Full Name hexosaminidase A (alpha polypeptide)
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 50-54 kDa, 60-67 kDa
GenBank accession numberBC018927
Gene symbol HEXA
Gene ID (NCBI) 3073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).