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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HIF-1 alpha Recombinant antibody (80933-1-RR)

    WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HIF-1 alpha Recombinant antibody (80933-1-RR)

    WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IHC staining of human colon using 80933-1-RR (same clone as 80933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IHC staining of human stomach using 80933-1-RR (same clone as 80933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IHC staining of human stomach cancer using 80933-1-RR (same clone as 80933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80933-1-RR (HIF-1 alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IHC staining of human thyroid cancer using 80933-1-RR (same clone as 80933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IHC staining of human thyroid cancer using 80933-1-RR (same clone as 80933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IF Staining of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594+phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIF-1 alpha Recombinant antibody (80933-1-RR)

    IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80933-1-RR

    Affinity and Kinetic Characterization of 80933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

HIF-1 alpha Recombinant antibody, PBS Only

HIF-1 alpha Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80933-1-PBS
Clone No.2K1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80933-1-PBS targets HIF-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIF-1 alpha fusion protein Ag15198 相同性解析による交差性が予測される生物種
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight120 kDa
GenBank accession numberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
RRIDAB_2918921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16665
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).