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HIF-1 alpha Recombinant antibody
HIF-1 alpha Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2K1
Cat no : 80933-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80933-1-RR targets HIF-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | HIF-1 alpha fusion protein Ag15198 相同性解析による交差性が予測される生物種 |
Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
Calculated molecular weight | 826 aa, 93 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC012527 |
Gene symbol | HIF1A |
Gene ID (NCBI) | 3091 |
RRID | AB_2918921 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).
Protocols
Product Specific Protocols | |
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WB protocol for HIF-1 alpha antibody 80933-1-RR | Download protocol |
IHC protocol for HIF-1 alpha antibody 80933-1-RR | Download protocol |
IF protocol for HIF-1 alpha antibody 80933-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |