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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIF2α/EPAS1 Monoclonal antibody, PBS Only

HIF2α/EPAS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6B12

Cat no : 66731-1-PBS

Synonyms

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HIF2α/EPAS1 Monoclonal antibody (66731-1-Ig)

    WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

    untreated and cobalt chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HIF2α/EPAS1 Monoclonal antibody (66731-1-Ig)

    WB analysis of HepG2 using 66731-1-Ig (same clone as 66731-1-PBS)

    untreated and cobalt chloride treated HepG2 cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIF2α/EPAS1 Monoclonal antibody (66731-1-Ig)

    IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIF2α/EPAS1 Monoclonal antibody (66731-1-Ig)

    IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66731-1-PBS targets HIF2α/EPAS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIF2α/EPAS1 fusion protein Ag24886 相同性解析による交差性が予測される生物種
Full Name endothelial PAS domain protein 1
Calculated molecular weight 96 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC051338
Gene symbol HIF2α
Gene ID (NCBI) 2034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.