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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIGD1A Monoclonal antibody, PBS Only (Capture)

HIGD1A Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A8E2

Cat no : 68231-1-PBS

Synonyms

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIGD1A Monoclonal antibody (68231-1-Ig)

    WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HIGD1A Monoclonal antibody (68231-1-Ig)

    IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50454-2

    Cytometric bead array standard curve of MP50454-2

    Cytometric bead array standard curve of MP50454-2, HIGD1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68231-1-PBS. Detection antibody: 68231-4-PBS. Standard:Ag14027. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68231-1-PBS targets HIGD1A as part of a matched antibody pair:

MP50454-2: 68231-1-PBS capture and 68231-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HIGD1A fusion protein Ag14027 相同性解析による交差性が予測される生物種
Full Name HIG1 hypoxia inducible domain family, member 1A
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC000601
Gene symbol HIGD1A
Gene ID (NCBI) 25994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.