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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIGD1A Monoclonal antibody (68231-1-Ig)

    WB analysis using 68231-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HIGD1A Monoclonal antibody (68231-1-Ig)

    IF Staining of U2OS using 68231-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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HIGD1A Monoclonal antibody

HIGD1A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68231-1-Ig
Clone No.3A8E2

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

DKFZp564K247, HIG1, HIG1 domain family member 1A, HIGD1A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, K-562 cells, human placenta tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68231-1-Ig targets HIGD1A in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HIGD1A fusion protein Ag14027 相同性解析による交差性が予測される生物種
Full Name HIG1 hypoxia inducible domain family, member 1A
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC000601
Gene Symbol HIGD1A
Gene ID (NCBI) 25994
RRIDAB_2935318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.

Protocols

Product Specific Protocols
WB protocol for HIGD1A antibody 68231-1-IgDownload protocol
IF protocol for HIGD1A antibody 68231-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols