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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated HIGD1A Monoclonal an (CL488-68231)

    IF Staining of U2OS using CL488-68231

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 HIGD1A antibody (CL488-68231, Clone: 3A8E2 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated HIGD1A Monoclonal an (CL488-68231)

    FC experiment of U2OS using CL488-68231

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody (CL488-68231, Clone:3A8E2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody

HIGD1A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68231
Clone No.3A8E2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68231 targets HIGD1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HIGD1A fusion protein Ag14027 相同性解析による交差性が予測される生物種
Full Name HIG1 hypoxia inducible domain family, member 1A
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC000601
Gene Symbol HIGD1A
Gene ID (NCBI) 25994
RRIDAB_3672981
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y241
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
FC protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
Standard Protocols
Click here to view our Standard Protocols