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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HINT2 Recombinant antibody (84031-5-RR)

    WB analysis using 84031-5-RR (same clone as 84031-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84031-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HINT2 Recombinant antibody (84031-5-RR)

    WB analysis of HeLa using 84031-5-RR (same clone as 84031-5-PBS)

    WB result of HINT2 antibody (84031-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT2 transfected HeLa cells. This data was developed using the same antibody clone with 84031-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HINT2 Recombinant antibody (84031-5-RR)

    IF Staining of HeLa using 84031-5-RR (same clone as 84031-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red). This data was developed using the same antibody clone with 84031-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84031-5-RR

    Affinity and Kinetic Characterization of 84031-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84031-5-RR against Human HINT2 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

HINT2 Recombinant antibody, PBS Only

HINT2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84031-5-PBS
Clone No.241150D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

241150D10, Adenosine 5'-monophosphoramidase HINT2, EC:3.9.1.-, HINT 2, HINT 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84031-5-PBS targets HINT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HINT2 fusion protein Ag12413 相同性解析による交差性が予測される生物種
Full Name histidine triad nucleotide binding protein 2
Calculated molecular weight 163 aa, 17 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC047737
Gene Symbol HINT2
Gene ID (NCBI) 84681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BX68
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.