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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HINT2 Recombinant antibody

HINT2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241150D10

Cat no : 84031-5-RR

Synonyms

HINT-2, HINT 3, HINT 2, EC:3.9.1.-, Adenosine 5'-monophosphoramidase HINT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HINT2 Recombinant antibody (84031-5-RR)

    WB analysis using 84031-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HINT2 Recombinant antibody (84031-5-RR)

    IF Staining of HeLa using 84031-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84031-5-RR

    Affinity and Kinetic Characterization of 84031-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84031-5-RR against Human HINT2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HuH-7 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84031-5-RR is HINT2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HINT2 fusion protein Ag12413 相同性解析による交差性が予測される生物種
Full Name histidine triad nucleotide binding protein 2
Calculated molecular weight 163 aa, 17 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC047737
Gene symbol HINT2
Gene ID (NCBI) 84681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HINT2 antibody 84031-5-RRDownload protocol
IF protocol for HINT2 antibody 84031-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols