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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D3G3

Cat no : CL594-67163

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated HIP1 Monoclonal antibody (CL594-67163)

    IF Staining of A549 using CL594-67163

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 HIP1 antibody (CL594-67163, Clone: 3D3G3 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67163 targets HIP1 in IF/ICC applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HIP1 fusion protein Ag17982 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1
Calculated molecular weight 1037 aa, 116 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC110545
Gene symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2920092
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

Protocols

Product Specific Protocols
IF protocol for CL594 HIP1 antibody CL594-67163Download protocol
Standard Protocols
Click here to view our Standard Protocols