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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HK3 Monoclonal antibody

HK3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3H3C7

Cat no : 67803-1-Ig

Synonyms

Hexokinase-C, Hexokinase-3, Hexokinase type III, hexokinase 3 (white cell), Hexokinase 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human saliva using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of human saliva using 67803-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of Raji using 67803-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of Ramos using 67803-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of RAW 264.7 using 67803-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using HK3 Monoclonal antibody (67803-1-Ig)

    WB analysis of rat spleen using 67803-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HK3 Monoclonal antibody (67803-1-Ig)

    IHC staining of human lung cancer using 67803-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HK3 Monoclonal antibody (67803-1-Ig)

    IHC staining of human lung cancer using 67803-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HK3 Monoclonal antibody (67803-1-Ig)

    IF Staining of HeLa using 67803-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HK3 antibody (67803-1-Ig, Clone: 3H3C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using HK3 Monoclonal antibody (67803-1-Ig)

    FC experiment of HeLa using 67803-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HK3 Monoclonal antibody (67803-1-Ig, Clone:3H3C7) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inRaji cells, Ramos cells, RAW 264.7 cells, human saliva, rat spleen tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67803-1-Ig targets HK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HK3 fusion protein Ag30798 相同性解析による交差性が予測される生物種
Full Name hexokinase 3 (white cell)
Calculated molecular weight 923 aa, 99 kDa
Observed molecular weight99 kDa
GenBank accession numberBC028129
Gene symbol HK3
Gene ID (NCBI) 3101
RRIDAB_2918566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

Protocols

Product Specific Protocols
WB protocol for HK3 antibody 67803-1-IgDownload protocol
IHC protocol for HK3 antibody 67803-1-IgDownload protocol
IF protocol for HK3 antibody 67803-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Biochem Biophys Res Commun

Identification of HK3 as a promising immunomodulatory and prognostic target in sepsis-induced acute lung injury

Authors - Mingyu Zhu
View Article