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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HK3 Monoclonal antibody

HK3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H3C7

Cat no : CL488-67803

Synonyms

Hexokinase-3, Hexokinase type III, hexokinase 3 (white cell), Hexokinase 3, EC:2.7.1.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HK3 Monoclonal antib (CL488-67803)

    IF Staining of HeLa using CL488-67803

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HK3 antibody (CL488-67803, Clone: 3H3C7 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated HK3 Monoclonal antib (CL488-67803)

    FC experiment of HeLa using CL488-67803

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Hk3 Monoclonal Antibody (CL488-67803, Clone:3H3C7)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67803 targets HK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HK3 fusion protein Ag30798 相同性解析による交差性が予測される生物種
Full Name hexokinase 3 (white cell)
Calculated molecular weight 923 aa, 99 kDa
Observed molecular weight99 kDa
GenBank accession numberBC028129
Gene symbol HK3
Gene ID (NCBI) 3101
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HK3 antibody CL488-67803Download protocol
FC protocol for CL Plus 488 HK3 antibody CL488-67803Download protocol
Standard Protocols
Click here to view our Standard Protocols