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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DMA Recombinant antibody

HLA-DMA Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230155E7

Cat no : 82899-2-RR

Synonyms

hCG_2001572, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, DMA protein, DMA, D6S222E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DMA Recombinant antibody (82899-2-RR)

    WB analysis using 82899-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82899-2-RR (HLA-DMA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using HLA-DMA Recombinant antibody (82899-2-RR)

    IHC staining of human colon using 82899-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using HLA-DMA Recombinant antibody (82899-2-RR)

    IHC staining of human colon using 82899-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82899-2-RR

    Affinity and Kinetic Characterization of 82899-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82899-2-RR against Human HLA-DMA were performed. The affinity constant is 0.303 nM.

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Tested Applications

Positive WB detected inDaudi cells, Ramos cells, Raji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82899-2-RR targets HLA-DMA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HLA-DMA fusion protein Ag2452 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DM alpha
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC026279
Gene symbol HLA-DMA
Gene ID (NCBI) 3108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

Protocols

Product Specific Protocols
WB protocol for HLA-DMA antibody 82899-2-RRDownload protocol
IHC protocol for HLA-DMA antibody 82899-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols