Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82899-2 targets HLA-DMA in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HLA-DMA fusion protein Ag2452 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DM alpha |
| Calculated molecular weight | 261 aa, 29 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC026279 |
| Gene Symbol | HLA-DMA |
| Gene ID (NCBI) | 3108 |
| RRID | AB_3673744 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q31604 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 HLA-DMA antibody CL750-82899-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |