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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant (65560-1-MR)

    FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant (65560-1-MR)

    FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

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Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody, PBS Only

HLA-DR Uni-rAbTM Recombinant Antibody for FC
Cat No. 65560-1-PBS
Clone No.L243

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65560-1-PBS targets HLA-DR in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01903
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.