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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L243

Cat no : 65560-1-MR

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Mouse Recombinant Antibo (65560-1-MR)

    FC experiment of human PBMCs using 65560-1-MR

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human HLA-DR Mouse Recombinant Antibody (65560-1-MR, Clone: L243) or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant (65560-1-MR)

    FC experiment of human PBMCs using 65560-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant (65560-1-MR)

    FC experiment of human PBMCs using 65560-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65560-1-MR targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for HLA-DR antibody 65560-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols