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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

L243

Cat no : Biotin-65560

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recom (Biotin-65560)

    FC experiment of human PBMCs using Biotin-65560

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (Biotin-65560, Clone: L243) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recom (Biotin-65560)

    FC experiment of human PBMCs using Biotin-65560

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (Biotin-65560, Clone: L243) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
RRIDAB_3672372
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).

Protocols

Product Specific Protocols
FC protocol for Biotin HLA-DR antibody Biotin-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols