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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

L243

Cat no : FITC-65560

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Re (FITC-65560)

    FC experiment of human PBMCs using FITC-65560

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 and 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Re (FITC-65560)

    FC experiment of human PBMCs using FITC-65560

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243) (red), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for FITC Plus HLA-DR antibody FITC-65560Download protocol
Standard Protocols
Click here to view our Standard Protocols