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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated HLA-E Monoclonal antibody

HLA-E Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A4G3

Cat no : CL594-66530

Synonyms

DKFZp686P19218, EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated HLA-E Monoclonal antibody (CL594-66530)

    IF Staining of human tonsillitis using CL594-66530

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-66530 (HLA-E antibody), at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated HLA-E Monoclonal antibody (CL594-66530)

    IF Staining of human tonsillitis using CL594-66530

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-66530 (HLA-E antibody), at dilution of 1:100.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66530 targets HLA-E in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HLA-E fusion protein Ag6724 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, E
Calculated molecular weight 40 kDa
GenBank accession numberBC002578
Gene symbol HLA-E
Gene ID (NCBI) 3133
RRIDAB_2883599
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Protocols

Product Specific Protocols
IF protocol for CL594 HLA-E antibody CL594-66530Download protocol
Standard Protocols
Click here to view our Standard Protocols