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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12C2

Cat no : 66819-1-Ig

Synonyms

HLA F antigen, HLA F, HLA CDA12, HLA 5.4, CDA12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-F Monoclonal antibody (66819-1-Ig)

    WB analysis using 66819-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using HLA-F Monoclonal antibody (66819-1-Ig)

    WB analysis of NCCIT using 66819-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-F Monoclonal antibody (66819-1-Ig)

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-F Monoclonal antibody (66819-1-Ig)

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-F Monoclonal antibody (66819-1-Ig)

    IF Staining of Raji using 66819-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-F antibody (66819-1-Ig, Clone: 1B12C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inA431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66819-1-Ig targets HLA-F in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6475 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC062991
Gene symbol HLA-F
Gene ID (NCBI) 3134
RRIDAB_2882162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
WB protocol for HLA-F antibody 66819-1-IgDownload protocol
IHC protocol for HLA-F antibody 66819-1-IgDownload protocol
IF protocol for HLA-F antibody 66819-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols