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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-F Monoclonal antibody, PBS Only

HLA-F Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12C2

Cat no : 66819-1-PBS

Synonyms

HLA F antigen, HLA F, HLA CDA12, HLA 5.4, CDA12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-F Monoclonal antibody (66819-1-Ig)

    WB analysis using 66819-1-Ig (same clone as 66819-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using HLA-F Monoclonal antibody (66819-1-Ig)

    WB analysis of NCCIT using 66819-1-Ig (same clone as 66819-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-F Monoclonal antibody (66819-1-Ig)

    IHC staining of human tonsillitis using 66819-1-Ig (same clone as 66819-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-F Monoclonal antibody (66819-1-Ig)

    IHC staining of human tonsillitis using 66819-1-Ig (same clone as 66819-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-F Monoclonal antibody (66819-1-Ig)

    IF Staining of Raji using 66819-1-Ig (same clone as 66819-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-F antibody (66819-1-Ig, Clone: 1B12C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66819-1-PBS targets HLA-F in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6475 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC062991
Gene symbol HLA-F
Gene ID (NCBI) 3134
RRIDAB_2882162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)