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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of U-937 using 66013-1-Ig (same clone as 66013-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HeLa using 66013-1-Ig (same clone as 66013-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HepG2 using 66013-1-Ig (same clone as 66013-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human white blood using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of human white blood using 66013-1-Ig (same clone as 66013-1-PBS)

    human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of pig spleen using 66013-1-Ig (same clone as 66013-1-PBS)

    pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of NCCIT using 66013-1-Ig (same clone as 66013-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IP experiment of HepG2 using 66013-1-Ig (same clone as 66013-1-PBS)

    IP result of anti-HLA class I ABC (IP:66013-1-Ig, 4ug; Detection:66013-1-Ig 1:4000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HLA class I ABC Monoclonal antibody, PBS Only

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, FC, IP, Indirect ELISA
Cat No. 66013-1-PBS
Clone No.5C5B7

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC, IP, Indirect ELISA

Synonyms

HLA-A, 5C5B7, HLA A, HLA class I, HLA class I (HLA-A)

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66013-1-PBS targets HLA class I ABC in WB, IHC, IF/ICC, FC, IP, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7488

Product name: Recombinant human HLA class I (HLA-A) protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-303 aa of BC003069

Sequence: SMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQ

相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_11042593
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04439
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

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