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  • Featured Product
  • KD/KO Validated

HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF/ICC, FC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5C5B7

Cat no : 66013-1-Ig

Synonyms

HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of U-937 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of U-937 using 66013-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of Raji using 66013-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis using 66013-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human white blood using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of human white blood using 66013-1-Ig

    human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HepG2 using 66013-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of pig spleen using 66013-1-Ig

    pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of NCCIT using 66013-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    WB analysis of HeLa using 66013-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human tonsillitis using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human liver cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IHC staining of human stomach cancer using 66013-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    IF Staining of Raji using 66013-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Raji cells using HLA class I ABC Monoclonal antibody (66013-1-Ig)

    FC experiment of Raji using 66013-1-Ig

    1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human white blood, NCCIT cells, pig spleen tissue, U-937 cells, Raji cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66013-1-Ig targets HLA class I ABC in WB, IHC, IF/ICC, FC, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_11042593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IHC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IF protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncotarget

Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells.

Authors - Jianlong Li
View Article
humanWB

J Tissue Eng Regen Med

Sequential hydrophile and lipophile solubilization as an efficient method for decellularization of porcine aortic valve leaflets: structure, mechanical property and biocompatibility study.

Authors - Wei-Hua Qiao
View Article
humanWB

J Biol Chem

The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.

Authors - Qi Wen Deng
  • KD Validated
View Article
pigIHC

Tissue Eng Part A

DECELLULARIZATION OF MASSIVE BONE ALLOGRAFTS BY PERFUSION: A NEW PROTOCOL FOR TISSUE ENGINEERING

Authors - Robin Evrard
View Article
humanIHC

Asian Pac J Cancer Prev

Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.

Authors - Zhao Xiaogang X
View Article
humanWB

Oncoimmunology

CD80 expression promotes immune surveillance in Barrett's metaplasia.

Authors - Melania Scarpa
View Article