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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated HLA class I ABC Monoclonal (CL594-66013)

    IF Staining of human tonsillitis using CL594-66013

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66013 (HLA class I ABC antibody) at dilution of 1:50.

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CoraLite®594-conjugated HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for IF-P
Cat No. CL594-66013
Clone No.5C5B7

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF-P

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66013 targets HLA class I ABC in IF-P applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_2883477
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04439
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
IF protocol for CL594 HLA class I ABC antibody CL594-66013Download protocol
Standard Protocols
Click here to view our Standard Protocols