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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for IF/ICC, IF-P, FC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, IF-P, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5C5B7

Cat no : CL488-66013

Synonyms

HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated HLA class I ABC Mono (CL488-66013)

    IF Staining of human tonsillitis using CL488-66013

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66013 (HLA class I ABC antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CoraLite® Plus 488-conjugated HLA class I ABC Mono (CL488-66013)

    IF Staining of Raji using CL488-66013

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 HLA class I ABC antibody (CL488-66013, Clone: 5C5B7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Raji cells using CoraLite® Plus 488-conjugated HLA class I ABC Mono (CL488-66013)

    FC experiment of Raji using CL488-66013

    1X10^6 Raji cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human HLA class I ABC (CL488-66013, Clone:5C5B7) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were not fixed.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue, Raji cells
Positive IF/ICC detected inRaji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66013 targets HLA class I ABC in IF/ICC, IF-P, FC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_2883227
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HLA class I ABC antibody CL488-66013Download protocol
Standard Protocols
Click here to view our Standard Protocols