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CoraLite® Plus 488-conjugated HLA class I ABC Monoclonal antibody
HLA class I ABC Monoclonal Antibody for IF/ICC, IF-P, FC
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
IF/ICC, IF-P, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5C5B7
Cat no : CL488-66013
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human tonsillitis tissue, Raji cells |
Positive IF/ICC detected in | Raji cells |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66013 targets HLA class I ABC in IF/ICC, IF-P, FC applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | HLA class I ABC fusion protein Ag7488 相同性解析による交差性が予測される生物種 |
Full Name | major histocompatibility complex, class I, A |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 40-44 kDa |
GenBank accession number | BC003069 |
Gene symbol | HLA-A |
Gene ID (NCBI) | 3105 |
RRID | AB_2883227 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 HLA class I ABC antibody CL488-66013 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |