CoraLite®555-conjugated HLA class I ABC Polyclonal antibody
HLA class I ABC Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-15240
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-15240 targets HLA class I ABC in IF/ICC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HLA class I ABC fusion protein Ag7370 相同性解析による交差性が予測される生物種 |
Full Name | major histocompatibility complex, class I, A |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 44 kDa |
GenBank accession number | BC003069 |
Gene symbol | HLA-A |
Gene ID (NCBI) | 3105 |
RRID | AB_2919626 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
Protocols
Product Specific Protocols | |
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IF protocol for CL555 HLA class I ABC antibody CL555-15240 | Download protocol |
FC protocol for CL555 HLA class I ABC antibody CL555-15240 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |