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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-15240

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated HLA class I ABC Polyclonal (CL555-15240)

    IF Staining of HepG2 using CL555-15240

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 HLA class I ABC antibody (CL555-15240) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-15240 targets HLA class I ABC in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7370 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_2919626
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
IF protocol for CL555 HLA class I ABC antibody CL555-15240Download protocol
FC protocol for CL555 HLA class I ABC antibody CL555-15240Download protocol
Standard Protocols
Click here to view our Standard Protocols