HMG20B Monoclonal antibody

HMG20B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C2A9

Cat no : 67354-1-Ig

Synonyms

BRAF25, BRAF35, BRCA2 associated factor 35, FLJ26127, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL


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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMG20B fusion protein Ag6103 相同性解析による交差性が予測される生物種
Full Name high-mobility group 20B
Calculated molecular weight 36 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002552
Gene symbol HMG20B
Gene ID (NCBI) 10362
RRIDAB_2882609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].

Protocols

Product Specific Protocols
WB protocol for HMG20B antibody 67354-1-IgDownload protocol
IHC protocol for HMG20B antibody 67354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols