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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMG20B Monoclonal antibody, PBS Only

HMG20B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C2A9

Cat no : 67354-1-PBS

Synonyms

BRAF25, BRAF35, BRCA2 associated factor 35, FLJ26127, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMG20B Monoclonal antibody (67354-1-Ig)

    WB analysis using 67354-1-Ig (same clone as 67354-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HMG20B Monoclonal antibody (67354-1-Ig)

    IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HMG20B Monoclonal antibody (67354-1-Ig)

    IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67354-1-PBS targets HMG20B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMG20B fusion protein Ag6103 相同性解析による交差性が予測される生物種
Full Name high-mobility group 20B
Calculated molecular weight 36 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002552
Gene symbol HMG20B
Gene ID (NCBI) 10362
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].