HMGB1 Monoclonal antibody

HMGB1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1D12A6

Cat no : 66525-1-Ig

Synonyms

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1


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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, L02 cells, K-562 cells
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66525-1-Ig targets HMGB1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMGB1 fusion protein Ag1264 相同性解析による交差性が予測される生物種
Full Name high-mobility group box 1
Calculated molecular weight 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003378
Gene symbol HMGB1
Gene ID (NCBI) 3146
RRIDAB_2881888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Protocols

Product Specific Protocols
WB protocol for HMGB1 antibody 66525-1-IgDownload protocol
IF protocol for HMGB1 antibody 66525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Nat Microbiol

Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis

Authors - Tomas Doyle
mouseWB

Adv Healthc Mater

Anti-PD-L1 DNAzyme Loaded Photothermal Mn 2+ /Fe 3+ Hybrid Metal-Phenolic Networks for Cyclically Amplified Tumor Ferroptosis-Immunotherapy

Authors - Peng Liu
mouse

J Control Release

Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy

Authors - Shiquan Sun
mouse

ACS Appl Mater Interfaces

Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy

Authors - Junmei Zhang
mouseWB

J Nanobiotechnology

Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reaction

Authors - Xue Li
human,mouseWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu