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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGB1 Monoclonal antibody, PBS Only

HMGB1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12A6

Cat no : 66525-1-PBS

Synonyms

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGB1 Monoclonal antibody (66525-1-Ig)

    WB analysis using 66525-1-Ig (same clone as 66525-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HMGB1 Monoclonal antibody (66525-1-Ig)

    WB analysis of Jurkat using 66525-1-Ig (same clone as 66525-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HMGB1 Monoclonal antibody (66525-1-Ig)

    WB analysis of HeLa using 66525-1-Ig (same clone as 66525-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HMGB1 Monoclonal antibody (66525-1-Ig)

    WB analysis of NIH/3T3 using 66525-1-Ig (same clone as 66525-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using HMGB1 Monoclonal antibody (66525-1-Ig)

    IF Staining of mouse lung using 66525-1-Ig (same clone as 66525-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using HMGB1 antibody (66525-1-Ig, Clone: 1D12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66525-1-PBS targets HMGB1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMGB1 fusion protein Ag1264 相同性解析による交差性が予測される生物種
Full Name high-mobility group box 1
Calculated molecular weight 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003378
Gene symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.