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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGCL Recombinant antibody (84769-4-RR)

    WB analysis using 84769-4-RR (same clone as 84769-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HMGCL Recombinant antibody (84769-4-RR)

    FC experiment of HeLa using 84769-4-RR (same clone as 84769-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84769-4-RR

    Affinity and Kinetic Characterization of 84769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

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HMGCL Recombinant antibody, PBS Only

HMGCL Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84769-4-PBS
Clone No.242224F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84769-4-PBS targets HMGCL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGCL fusion protein Ag10426 相同性解析による交差性が予測される生物種
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated molecular weight 325 aa, 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC010570
Gene Symbol HMGCL
Gene ID (NCBI) 3155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35914
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.