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HMGCL Recombinant antibody

HMGCL Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84769-4-RR
Clone No.242224F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 ug/ml
20 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84769-4-RR targets HMGCL in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGCL fusion protein Ag10426 相同性解析による交差性が予測される生物種
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated molecular weight 325 aa, 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC010570
Gene Symbol HMGCL
Gene ID (NCBI) 3155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

Protocols

Product Specific Protocols
WB protocol for HMGCL antibody 84769-4-RRDownload protocol
FC protocol for HMGCL antibody 84769-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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