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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNMT Recombinant antibody, PBS Only

HNMT Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242159E3

Cat no : 84777-3-PBS

Synonyms

HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNMT Recombinant antibody (84777-3-RR)

    WB analysis using 84777-3-RR (same clone as 84777-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84777-3-RR (HNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84777-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84777-3-RR

    Affinity and Kinetic Characterization of 84777-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84777-3-RR against Human HNMT were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84777-3-PBS targets HNMT in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNMT fusion protein Ag2462 相同性解析による交差性が予測される生物種
Full Name histamine N-methyltransferase
Calculated molecular weight 292 aa, 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC020677
Gene symbol HNMT
Gene ID (NCBI) 3176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.