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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNMT Recombinant antibody

HNMT Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242159E3

Cat no : 84777-3-RR

Synonyms

HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNMT Recombinant antibody (84777-3-RR)

    WB analysis using 84777-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84777-3-RR (HNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84777-3-RR

    Affinity and Kinetic Characterization of 84777-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84777-3-RR against Human HNMT were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84777-3-RR targets HNMT in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNMT fusion protein Ag2462 相同性解析による交差性が予測される生物種
Full Name histamine N-methyltransferase
Calculated molecular weight 292 aa, 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC020677
Gene symbol HNMT
Gene ID (NCBI) 3176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

Protocols

Product Specific Protocols
WB protocol for HNMT antibody 84777-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols