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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis using 11760-1-AP (same clone as 11760-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of human brain using 11760-1-AP (same clone as 11760-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using HNRNPC Polyclonal antibody (11760-1-AP)

    WB analysis of K-562 using 11760-1-AP (same clone as 11760-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    IP experiment of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    IP result of anti-HNRNPC (IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of human lymphoma using 11760-1-AP (same clone as 11760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11760-1-AP (HNRNPC antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPC Polyclonal antibody (11760-1-AP)

    IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPC Polyclonal antibody (11760-1-AP)

    IF Staining of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HNRNPC Polyclonal antibody, PBS Only

HNRNPC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11760-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11760-1-PBS targets HNRNPC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPC fusion protein Ag2356 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa, 36-40 kDa
GenBank accession numberBC003394
Gene Symbol HNRNPC
Gene ID (NCBI) 3183
RRIDAB_2117500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07910
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.