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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555-conjugated HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

1A6C2

Cat no : CL555-67375

Synonyms

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 555-conjugated HNRNPH1 Monoclonal a (CL555-67375)

    IF Staining of HepG2 using CL555-67375

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 HNRNPH1 antibody (CL555-67375, Clone: 1A6C2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67375 targets HNRNPH1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HNRNPH1 fusion protein Ag6603 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein H1 (H)
Calculated molecular weight 49 kDa
Observed molecular weight 49-50 kDa
GenBank accession numberBC001348
Gene symbol HNRNPH1
Gene ID (NCBI) 3187
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Protocols

Product Specific Protocols
IF protocol for CL Plus 555 HNRNPH1 antibody CL555-67375Download protocol
Standard Protocols
Click here to view our Standard Protocols