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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPL Polyclonal antibody (18354-1-AP)

    WB analysis using 18354-1-AP (same clone as 18354-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    WB analysis of mouse liver using 18354-1-AP (same clone as 18354-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using HNRNPL Polyclonal antibody (18354-1-AP)

    IP experiment of BxPC-3 using 18354-1-AP (same clone as 18354-1-PBS)

    IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HNRNPL Polyclonal antibody (18354-1-AP)

    IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPL Polyclonal antibody (18354-1-AP)

    IF Staining of HeLa using 18354-1-AP (same clone as 18354-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPL antibody (18354-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HNRNPL Polyclonal antibody, PBS Only

HNRNPL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18354-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

P/OKcl.14, HNRPL, hnRNP L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18354-1-PBS targets HNRNPL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPL fusion protein Ag13211 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein L
Calculated molecular weight 572 aa, 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC069184
Gene Symbol HNRNPL
Gene ID (NCBI) 3191
RRIDAB_2878537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14866
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.