Validation Data Gallery

Filter: AllWBIHCIF-PFC (Intra)

WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)
WB result of HO-1/HMOX1 antibody (66743-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1/HMOX1 transfected HeLa cells. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66743-1-Ig (same clone as 66743-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
IF Staining of rat liver using 66743-1-Ig (same clone as 66743-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.4 ug HO-1/HMOX1 Monoclonal antibody (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.4 ug HO-1/HMOX1 Monoclonal antibody (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HO-1/HMOX1 Monoclonal antibody, PBS Only

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 66743-1-PBS

Clone No.2D10A5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66743-1-PBS targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	HO-1/HMOX1 fusion protein Ag21296 相同性解析による交差性が予測される生物種
Full Name	heme oxygenase (decycling) 1
Calculated molecular weight	33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC001491
Gene Symbol	HO-1
Gene ID (NCBI)	3162
RRID	AB_2882091
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P09601
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human renal cell carcinoma using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human kidney using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human kidney using 66743-1-Ig (same clone as 66743-1-PBS)

IHC staining of human renal cell carcinoma using 66743-1-Ig (same clone as 66743-1-PBS)

IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)

IF Staining of rat liver using 66743-1-Ig (same clone as 66743-1-PBS)

FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

HO-1/HMOX1 Monoclonal antibody, PBS Only

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information