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HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 66743-1-Ig
Clone No.2D10A5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, rat liver tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, pig, sheep
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag21296 相同性解析による交差性が予測される生物種
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001491
Gene Symbol HO-1
Gene ID (NCBI) 3162
RRIDAB_2882091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IHC protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
IF protocol for HO-1/HMOX1 antibody 66743-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Dev Cell

HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis

Authors - Xiang Peng
WB

Adv Healthc Mater

Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing.

Authors - Qiuyue Ding
mouseWB

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
humanWB,IHC,IP

Oncogene

The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1.

Authors - Ming Gao
mouseWB,IF

EMBO Rep

Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy

Authors - Jie Li
humanWB

Cell Biosci

Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth

Authors - Hongting Zhao
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