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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10A5

Cat no : CL594-66743

Synonyms

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 Monoclonal anti (CL594-66743)

    IF Staining of human liver using CL594-66743

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite®594-conjugated HO-1/HMOX1 Monoclonal anti (CL594-66743)

    IF Staining of mouse spleen using CL594-66743

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®594 HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 Monoclonal anti (CL594-66743)

    IF Staining of human liver using CL594-66743

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated HO-1/HMOX1 Monoclonal anti (CL594-66743)

    FC experiment of HeLa using CL594-66743

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HO-1/HMOX1 (CL594-66743, Clone:2D10A5) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse spleen tissue, human liver tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag21296 相同性解析による交差性が予測される生物種
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001491
Gene symbol HO-1
Gene ID (NCBI) 3162
RRIDAB_2883623
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
IF protocol for CL594 HO-1/HMOX1 antibody CL594-66743Download protocol
FC protocol for CL594 HO-1/HMOX1 antibody CL594-66743Download protocol
Standard Protocols
Click here to view our Standard Protocols