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HORMAD1 Recombinant antibody

HORMAD1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84003-5-RR
Clone No.241117E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman testis tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84003-5-RR targets HORMAD1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HORMAD1 fusion protein Ag4914 相同性解析による交差性が予測される生物種
Full Name HORMA domain containing 1
Calculated molecular weight 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC047406
Gene Symbol HORMAD1
Gene ID (NCBI) 84072
RRIDAB_3671574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86X24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].

Protocols

Product Specific Protocols
WB protocol for HORMAD1 antibody 84003-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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