Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66798 targets HPGD in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HPGD fusion protein Ag1499 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxyprostaglandin dehydrogenase 15-(NAD) |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC018986 |
| Gene Symbol | HPGD |
| Gene ID (NCBI) | 3248 |
| RRID | AB_2920040 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15428 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HPGD antibody CL594-66798 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |