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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HPRT1 Polyclonal antibody

HPRT1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15059

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HPRT1 Polyclonal ant (CL488-15059)

    IF Staining of HeLa using CL488-15059

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HPRT1 antibody (CL488-15059) at dilution of 1:200, CL594-Phalloidin (red).

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15059 targets HPRT1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPRT1 fusion protein Ag7044 相同性解析による交差性が予測される生物種
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated molecular weight 25 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC000578
Gene symbol HPRT1
Gene ID (NCBI) 3251
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HPRT1 antibody CL488-15059Download protocol
Standard Protocols
Click here to view our Standard Protocols