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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HEK-293 using 60069-1-Ig

    WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

  • Western Blot (WB) analysis of ROS1728 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of ROS1728 using 60069-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HEK-293 using 60069-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HeLa using 60069-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of Jurkat using 60069-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of RAW 264.7 using 60069-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERAB Monoclonal antibody (60069-1-Ig)

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERAB Monoclonal antibody (60069-1-Ig)

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

ERAB Monoclonal antibody

ERAB Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60069-1-Ig
Clone No.1G5E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inROS1728 cells, RAW 264.7 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60069-1-Ig targets ERAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERAB fusion protein Ag1020 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 10
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC008708
Gene Symbol ERAB
Gene ID (NCBI) 3028
RRIDAB_2119779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99714
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

Protocols

Product Specific Protocols
WB protocol for ERAB antibody 60069-1-IgDownload protocol
IHC protocol for ERAB antibody 60069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols