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  • Featured Product
  • KD/KO Validated

ERAB Monoclonal antibody

ERAB Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5E5

Cat no : 60069-1-Ig

Synonyms

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HEK-293 using 60069-1-Ig

    WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

  • Western Blot (WB) analysis of ROS1728 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of ROS1728 using 60069-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HEK-293 using 60069-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of HeLa using 60069-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of Jurkat using 60069-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using ERAB Monoclonal antibody (60069-1-Ig)

    WB analysis of RAW 264.7 using 60069-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERAB Monoclonal antibody (60069-1-Ig)

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERAB Monoclonal antibody (60069-1-Ig)

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inROS1728 cells, RAW 264.7 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60069-1-Ig targets ERAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERAB fusion protein Ag1020 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 10
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC008708
Gene symbol ERAB
Gene ID (NCBI) 3028
RRIDAB_2119779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

Protocols

Product Specific Protocols
WB protocol for ERAB antibody 60069-1-IgDownload protocol
IHC protocol for ERAB antibody 60069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols