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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HSDL2 Polyclonal ant (CL488-15631)

    IF Staining of HeLa using CL488-15631

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HSDL2 antibody (CL488-15631) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HSDL2 Polyclonal antibody

HSDL2 Polyclonal Antibody for IF/ICC
Cat No. CL488-15631

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

C9orf99, FLJ25855, HSDL2, SDR13C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15631 targets HSDL2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSDL2 fusion protein Ag7987 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid dehydrogenase like 2
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC004331
Gene Symbol HSDL2
Gene ID (NCBI) 84263
RRIDAB_3672635
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YN16
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSDL2 antibody CL488-15631Download protocol
Standard Protocols
Click here to view our Standard Protocols